Interferon‐γ binds to heparan sulfate by a cluster of amino acids located in the C‐terminal part of the molecule
- 11 March 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 280 (1) , 152-154
- https://doi.org/10.1016/0014-5793(91)80225-r
Abstract
Using three different approaches (domain mapping with monoclonal antibodies, limited enzymatic digestion and competition with synthetic peptides), we demonstrated that a cluster of basic amino acids on interferon-gamma is involved in its binding to heparan sulfate. This cluster (Lys125-Arg131) is localized in the C-terminal part of IFN-gamma. Once bound to heparin sulfate, IFN-gamma is protected against protease attack.Keywords
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