TRANSIT PATTERNS OF MARKER COMPOUNDS GIVEN BY SEGMENTED RETROGRADE INTRABILIARY INJECTION (SRII) IN THE ISOLATED INSITU PERFUSED-RAT-LIVER

  • 1 January 1980
    • journal article
    • research article
    • Vol. 215  (1) , 110-115
Abstract
The mode of transfer of solutes across the biliary tree epithelium in the reabsorptive direction was studied by the segmented retrograde intrabiliary injection technique in isolated perfused liver of rats. The main purpose was to study the pattern of transit of the radioactive marker compounds through the liver into the venous perfusate. The perfusate was collected as a single pass. All marker compounds were quantitatively accounted for in the bile and the perfusate. The apparent volumes of distribution of dextran and sucrose were smaller than that for erythritol. Mannitol was similar to erythritol. The mean transit times were correspondingly: dextran .simeq. sucrose < mannitol .simeq. erythritol. Dehydrocholate (100 .mu.mol/kg) had no effect on these parameters, but taurocholate (100 .mu.mol/kg) increased the mean transit time for sucrose. This effect was probably related to an increase in biliary tree permeability produced by taurocholate. The present approach appeared to be useful in assessing hepatobiliary function and its alteration by allowing the transit patterns of compounds to be determined in passing from bile through the liver to the blood.