Tumor induction by Agrobacterium rhizogenes involves the transfer of plasmid DNA to the plant genome
Open Access
- 1 May 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (10) , 3193-3197
- https://doi.org/10.1073/pnas.79.10.3193
Abstract
The DNA from tumors of Nicotiana glauca initiated by strains of A. rhizogenes contained sequences that are homologous to the root-inducing (Ri) plasmid of the bacterium. Two independently established tumor lines contained a similar portion of the Ri-plasmid. The Ri-plasmid also hybridized to DNA fragments from uninfected N. glauca. A cosmid clone of the Ri-plasmid encompassing the region containing the Ri-plasmid sequences that are stably transferred to the plant also hybridized to the Ri-plasmid-related fragments found in uninfected plants. Of 6 tumor lines tested, 5 produced a tumor-specific compound that is similar to agropine.Keywords
This publication has 27 references indexed in Scilit:
- Recombination between higher plant DNA and the Ti plasmid of Agrobacterium tumefaciensProceedings of the National Academy of Sciences, 1980
- A small cosmid for efficient cloning of large DNA fragmentsGene, 1980
- Tumor DNA Structure in Plant Cells Transformed by A. tumefaciensScience, 1980
- Foreign DNA sequences in crown gall teratomas and their fate during the loss of the tumorous traitsMolecular Genetics and Genomics, 1980
- Integration and organization of Ti plasmid sequences in crown gall tumorsCell, 1980
- Involvement of a plasmid in the hairy root disease of plants caused by Agrobacterium rhizogenesPlasmid, 1979
- Cloning Human Fetal γ Globin and Mouse α-Type Globin DNA: Preparation and Screening of Shotgun CollectionsScience, 1978
- Isolation of covalently closed circular DNA of high molecular weight from bacteriaAnalytical Biochemistry, 1976
- [ON THE PRESENCE OF OCTINE IN CROWN-GALL].1964
- Detection of Sugars on Paper ChromatogramsNature, 1950