Fast reconstruction of four-dimensional NMR spectra from plane projections
- 1 April 2004
- journal article
- Published by Springer Nature in Journal of Biomolecular NMR
- Vol. 28 (4) , 391-395
- https://doi.org/10.1023/b:jnmr.0000015421.60023.e5
Abstract
Four-dimensional NMR spectroscopy can be speeded up by a large factor by a projection-reconstruction technique related to that used in X-ray tomography. The limited amount of information recorded in a few suitably-tilted projection planes suffices to recreate the entire four-dimensional spectrum. The method is demonstrated by reference to the 500 MHz spectrum of 1 millimolar aqueous solution of ubiquitin by dispersing the responses of the three-dimensional HNCO spectrum into the Cα dimension of a four-dimensional HNCOCA spectrum obtained by projection-reconstruction.Keywords
This publication has 8 references indexed in Scilit:
- Projection−Reconstruction of Three-Dimensional NMR SpectraJournal of the American Chemical Society, 2003
- GFT NMR, a New Approach To Rapidly Obtain Precise High-Dimensional NMR Spectral InformationJournal of the American Chemical Society, 2003
- Multiple quadrature detection in reduced dimensionality experiments.Journal of Biomolecular NMR, 2003
- New methods for fast multidimensional NMRJournal of Biomolecular NMR, 2003
- Reconstruction of the three-dimensional NMR spectrum of a protein from a set of plane projectionsJournal of Biomolecular NMR, 2003
- Reduced-dimensionality NMR spectroscopy for high-throughput protein resonance assignmentProceedings of the National Academy of Sciences, 2002
- Novel 2D Triple-Resonance NMR Experiments for Sequential Resonance Assignments of ProteinsJournal of Magnetic Resonance, 2002
- Computerized transverse axial scanning (tomography): Part 1. Description of systemThe British Journal of Radiology, 1973