Plasmid Profile Analysis ofPasteurella piscicidaand Use of a Plasmid DNA Probe to Identify the Species

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Abstract
The plasmid profiles of 23 isolates of Pasteurella piscicida, isolated from cultured buri Seriola quinqueradiata in Japan, were analyzed by agarose gel electrophoresis. Most isolates possessed one large plasmid that was 110 kilobase pairs (kb) in size and two small plasmids that were 3.5 and 5.1 kb in size. The 5.1‐kb plasmid was common to all 23 isolates from Japan. However, this plasmid was not found in the related organisms Pasteurella haemolytica, P. multocida, and Haemophilus influenzae, or in other bacteria that are pathogenic to fish (Aeromonas hydrophila, A. salmonicida, Edwardsiella tarda, Pseudomonas anguilliseptica, Vibrio anguillarum, and Yersinia ruckeri). The 5.1‐kb plasmid was used as a probe for the rapid detection and identification of P. piscicida by hybridization methods.

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