Enzymatic Conversion ofd-Glucose tod-Fructose

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Abstract
Propagation of S. phaeochromogenus by both shaking and submerged cultivations is described. The addition of Co++ to medium remarkably improved the activity of the cell to convert glucose into fructose. The cell grown with Co++ isomerized glucose at pH 9.0 and 7.5 similarly, whereas the cells grown without Co++ reacted with glucose at pH 9.3 as the optimum. In submerged cultivation, the mode of pH change during cultivation period varied depending on the kind of strain, carbon source and the presence of Co++. In the propagation of the strain SK, carbon and nitrogen sources were rather rapidly consumed resulting in cell growth, when pH value of the medium was falling. On the other hand, however, growth hardly developed at the pH values lower than 6.0. Therefore, it was necessary to adjust pH with alkali in order to get active cell yield.

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