Elderberry (Sambucus nigra) contains truncated Neu5Ac(α‐2,6)Gal/GalNAc‐binding type 2 ribosome‐inactivating proteins
- 20 March 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 425 (1) , 35-39
- https://doi.org/10.1016/s0014-5793(98)00193-8
Abstract
Analysis of affinity‐purified preparations of the fetuin‐binding proteins from elderberry bark and fruits revealed besides the previously reported Neu5Ac(α‐2,6)Gal/GalNAc‐specific type 2 ribosome‐inactivating proteins (RIP) the occurrence of single chain proteins of 22 kDa, which according to their N‐terminal amino acid sequence correspond to the second part of the B chain of the respective type 2 RIP. Both proteins are very similar except that the polypeptides of the fruit lectin are 10 amino acid residues longer than these from the bark lectin. Our findings not only demonstrate the occurrence of carbohydrate‐binding fragments of type 2 RIP but also provide further evidence that type 2 RIP genes give rise to complex mixtures of type 2 RIP/lectins in elderberry.Keywords
This publication has 18 references indexed in Scilit:
- Elderberry (Sambucus Nigra) Bark Contains two Structurally Different Neusac(α2,6)Gal/Galnac‐Binding Type 2 Ribosome‐Inactivating ProteinsEuropean Journal of Biochemistry, 1997
- Isolation and Molecular Cloning of a Novel Type 2 Ribosome-inactivating Protein with an Inactive B Chain from Elderberry (Sambucus nigra) BarkJournal of Biological Chemistry, 1997
- Polynucleotide:adenosine glycosidase activity of ribosome-inactivating proteins: effect on DNA, RNA and poly(A)Nucleic Acids Research, 1997
- Isolation and characterization of a new non-toxic two-chain ribosome-inactivating protein from fruits of elder (Sambucus nigraL.)Journal of Experimental Botany, 1996
- A non-toxic two-chain ribosome-inactivating protein co-exists with a structure-related monomeric lectin (SNA III) in elder (Sambucus nigra) fruitsBiochemical Journal, 1996
- Characterization and Molecular Cloning of Sambucus nigra Agglutinin V (Nigrin b), A Galnac‐specific Type‐2 Ribosome‐Inactivating Protein from the Bark of Elderberry (Sambucus nigra)European Journal of Biochemistry, 1996
- The NeuAc(α‐2,6)‐Gal/GalNAc‐Binding Lectin from Elderberry (Sambucus Nigra) Bark, a type‐2 Ribosome‐Inactivating Protein with an Unusual Specificity and StructureEuropean Journal of Biochemistry, 1996
- Lectins as Plant Defense ProteinsPlant Physiology, 1995
- Elderberry (Sambucus nigraL.) seed proteins inhibit protein synthesis and display strong immunoreactivity with rabbit polyclonal antibodies raised against the type 2 ribosome-inactivating protein nigrin bJournal of Experimental Botany, 1994
- Ribosome-inactivating proteins from plantsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1993