Flow Cytometric Determination of Nuclear Replication Stage in Seed Tissues

1 August 1993

journal article
Published by Oxford University Press (OUP) in Annals of Botany

Vol. 72 (2) , 181-187
https://doi.org/10.1006/anbo.1993.1097

Abstract

Flow cytometric determination of DNA levels in embryos of fully matured seeds of various plant species revealed large amounts of 2C DNA signals, indicating that most cells had arrested the cell cycle at the presynthetic G₁ phase of nuclear division. The accumulation of cells at G₁ was found both in orthodox and in recalcitrant (i.e. Castanea sativa) seed species. As recalcitrant seeds are characterized by the absence of maturation drying, the arrest of the cell cycle in the presynthetic phase may not be linked to the seed water status.

Keywords

NUCLEAR REPLICATION
SEED TISSUES
REPLICATION STAGE
FLOW CYTOMETRIC DETERMINATION

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