Studies on Nuclear Acid Deoxyribonucleases and Proofreading Exonuclease Isolated from Rat Liver Nuclei
- 1 October 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 104 (4) , 521-525
- https://doi.org/10.1093/oxfordjournals.jbchem.a122503
Abstract
Three kinds of deoxyribonucleases (peaks A, B, and C) were separated from purified rat liver nuclei on DEAE-cellulose column chromatography, and their characteristics were partially studied. Peaks A and B had endonuclease activities under acidic conditions with low substrate specificity and did not require divalent metal ions. Peak C had an exonuclease activity under alkaline conditions with substrate specificity for denatured DNA or single stranded homopolymer and required divalent cations. Peak C degraded 3′-terminally mismatched substrate much faster than 3′-terminally matched substrate.Keywords
This publication has 15 references indexed in Scilit:
- A separate editing exonuclease for DNA replication: the epsilon subunit of Escherichia coli DNA polymerase III holoenzyme.Proceedings of the National Academy of Sciences, 1984
- Formation of deoxyribonucleoside 5′-monophosphates dependent on DNA synthesis in nuclei isolated from regenerating rat liverBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1984
- Conversion of dNTP to dNMP dependent on DNA synthesis in isolated Yoshida sarcoma nucleiBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1982
- Restricted Hydrolysis of Newly Replicated DNA in Isolated Nuclei from Yosbida Sarcoma CellsThe Journal of Biochemistry, 1981
- On the fidelity of DNA replication. Nucleoside monophosphate generation during polymerization.Journal of Biological Chemistry, 1981
- Mechanism of primer-template-dependent conversion of dNTP leads to dNMP by T5 DNA polymerase.Journal of Biological Chemistry, 1980
- Selective inhibition of the 3' to 5' exonuclease activity associated with DNA polymerases: a mechanism of mutagenesisBiochemistry, 1977
- A new mammalian DNA polymerase with 3' to 5' exonuclease activity: DNA polymerase δBiochemistry, 1976
- The effect of fluoride on succinic oxidase systemBiochemical Journal, 1952
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951