A physical map of the Myxococcus xanthus chromosome.
- 27 September 1994
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 91 (20) , 9584-9587
- https://doi.org/10.1073/pnas.91.20.9584
Abstract
A physical map of the 9.2-Mbp Myxococcus xanthus DK1622 chromosome at a resolution of 25 kbp was constructed by using a strategy that is applicable to virtually all microorganisms. Segments of the chromosome were used as hybridization probes to subdivide a yeast artificial chromosome (YAC) library into groups of linked clones. The clones were aligned by comparing their EcoRI restriction patterns. The groups of YAC clones ("contigs") were oriented and aligned with the genomic restriction map by means of common genetic and physical markers such as rare restriction sites and transposon insertions. Over 95% of the genome is represented by cloned DNA. Sixty genetic loci including > 100 genes, many of which play a role in fruiting body development, have been mapped in this way. Additional genes can now be located on the chromosome map by hybridization of their sequences to the ordered set of YAC chromosomes. The mapped genetic loci account for approximately 2% of the genome.Keywords
This publication has 20 references indexed in Scilit:
- A physical and genetic map of the Stigmatella aurantiaca DW4/3.1 chromosomeMolecular Microbiology, 1993
- Mutation and mapping of genes involved in production of the antibiotic TA in Myxococcus xanthusAntimicrobial Agents and Chemotherapy, 1992
- Physical mapping of the Myxococcus xanthus genome by random cloning in yeast artificial chromosomes.Proceedings of the National Academy of Sciences, 1989
- The physical map of the whole E. coli chromosome: Application of a new strategy for rapid analysis and sorting of a large genomic libraryCell, 1987
- Cloning of Large Segments of Exogenous DNA into Yeast by Means of Artificial Chromosome VectorsScience, 1987
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry, 1983
- Regions of DNA involved in the stringent control of plasmid-encoded rRNA in vivoCell, 1983
- Mode of action of Myxococcus xanthus antibiotic TAAntimicrobial Agents and Chemotherapy, 1981
- Structural Analysis of Tn5Published by Cold Spring Harbor Laboratory ,1981
- A colony bank containing synthetic CoI EI hybrid plasmids representative of the entire E. coli genomeCell, 1976