Differential effects of apolipoprotein E isoforms on phosphorylation at specific sites on tau by glycogen synthase kinase‐3β identified by nano‐electrospray mass spectrometry
- 21 November 2000
- journal article
- Published by Wiley in FEBS Letters
- Vol. 485 (2-3) , 99-103
- https://doi.org/10.1016/s0014-5793(00)02196-7
Abstract
Previously published data have shown an allele-specific variation in the in vitro binding of apolipoprotein E (apoE) to tau, which prompted the hypothesis that apoE binding may protect tau from phosphorylation, apoE3 being more efficient than apoE4. We have, therefore, investigated the effects of apoE on tau phosphorylation in vitro by the proline-directed kinase, glycogen synthase kinase (GSK)-3β. The phosphopeptide maps of tau alone, of tau with apoE3 and of tau with apoE4 were very similar. When apoE2 was present a further four spots were evident. Additionally, of the 15 peptides phosphorylated in the presence or absence of apoE, subtle differences, some isoform-specific, in the relative amounts of phosphorylation were observed.Keywords
This publication has 38 references indexed in Scilit:
- Expression of Human Apolipoprotein E4 in Neurons Causes Hyperphosphorylation of Protein Tau in the Brains of Transgenic MiceThe American Journal of Pathology, 2000
- Phosphorylation of tau protein by recombinant GSK‐3β: pronounced phosphorylation at select Ser/Thr‐Pro motifs but no phosphorylation at Ser262 in the repeat domainFEBS Letters, 1999
- Sequential phosphorylation of Tau by glycogen synthase kinase‐3β and protein kinase A at Thr212 and Ser214 generates the Alzheimer‐specific epitope of antibody AT100 and requires a paired‐helical‐filament‐like conformationEuropean Journal of Biochemistry, 1998
- Analytical Properties of the Nanoelectrospray Ion SourceAnalytical Chemistry, 1996
- Apolipoprotein E immunoreactivity within neurofibrillary tangles: relationship to tau and PHF in Alzheimer's diseaseExperimental Neurology, 1995
- Alzheimer's disease-like phosphorylation of the microtubule-associated protein tau by glycogen synthase kinase-3 in transfected mammalian cellsCurrent Biology, 1994
- PHF‐tau from Alzheimer's brain comprises four species on SDS‐PAGE which can be mimicked by in vitro phosphorylation of human brain tau by glycogen synthase kinase‐3βFEBS Letters, 1994
- Stiff microtubules and neuronal morphologyTrends in Neurosciences, 1994
- Gene Dose of Apolipoprotein E Type 4 Allele and the Risk of Alzheimer's Disease in Late Onset FamiliesScience, 1993
- The Alzheimer‐like phosphorylation of tau protein reduces microtubule binding and involves Ser‐Pro and Thr‐Pro motifsFEBS Letters, 1992