The Arabidopsis FLC protein interacts directly in vivo with SOC1 and FT chromatin and is part of a high‐molecular‐weight protein complex
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Open Access
- 21 March 2006
- journal article
- Published by Wiley in The Plant Journal
- Vol. 46 (2) , 183-192
- https://doi.org/10.1111/j.1365-313x.2006.02686.x
Abstract
Summary: The Arabidopsis Flowering Locus C (FLC) protein is a repressor of flowering regulated by genes in the autonomous and vernalization pathways. Previous genetic and transgenic data have suggested that FLC acts by repressing expression of the floral integrator genes SOC1 and FT. We have taken an in vivo approach to determine whether the FLC protein interacts directly with potential DNA targets. Using chromatin immunoprecipitation, we have shown that FLC binds to a region of the first intron of FT that contains a putative CArG box, and have confirmed that FLC binds to a CArG box in the promoter of the SOC1 gene. MADS box proteins are thought to bind their DNA targets as dimers or higher‐order multimers. We have shown that FLC is a component of a multimeric protein complex in vivo and that more than one FLC polypeptides can be present in the complex.Keywords
This publication has 30 references indexed in Scilit:
- Role of FRIGIDA and FLOWERING LOCUS C in Determining Variation in Flowering Time of ArabidopsisPlant Physiology, 2005
- Comprehensive Interaction Map of the Arabidopsis MADS Box Transcription FactorsPlant Cell, 2005
- Analysis of the ArabidopsisMADS AFFECTING FLOWERINGGene Family:MAF2Prevents Vernalization by Short Periods of Cold [W]Plant Cell, 2003
- FLC, a repressor of flowering, is regulated by genes in different inductive pathwaysThe Plant Journal, 2002
- High-throughput vectors for efficient gene silencing in plantsFunctional Plant Biology, 2002
- A MADS domain gene involved in the transition to flowering in ArabidopsisThe Plant Journal, 2000
- The MADS-Box Family of Transcription FactorsEuropean Journal of Biochemistry, 1995
- The late‐flowering phenotype of FRIGIDA and mutations in LUMINIDEPENDENS is suppressed in the Landsberg erecta strain of ArabidopsisThe Plant Journal, 1994
- The phenotype of some late‐flowering mutants is enhanced by a locus on chromosome 5 that is not effective in the Landsberg erecta wild‐typeThe Plant Journal, 1994
- Nucleotide sequences recognized by the AGAMOUS MADS domain of Arabidopsis thaliana in vitroThe Plant Journal, 1993