Immobilization ofmyo-inositol-1-phosphate synthase containing active, self-regenerating coenzyme (NAD+) on the same matrix
- 1 June 1981
- journal article
- Published by Springer Nature in Applied Biochemistry and Biotechnology
- Vol. 6 (2) , 85-90
- https://doi.org/10.1007/bf02779242
Abstract
myo-Inositol-1-phosphate synthase (EC 5.5.1.4) from rat testes, an NAD+-containing enzyme, which convertsd-glucose 6-phosphate to 1l-myo-inositol 1-phosphate, could be immobilized together with its cofactor and bovine serum albumin by crosslinking with glutaraldehyde at pH 4.5. The enzyme bound to the gel showed a specific activity of 5.6% of that of the native enzyme, but the activity could be increased to 21% by pretreatment with urea.Keywords
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