Inhibition of p-aminohippurate transport by cyclic GMP in rat kidney cortical slices.

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Abstract
The effect of cGMP on the accumulation of p-aminohippurate (PAH) was investigated using rat kidney cortical slices. When the slices were preincubated with cGMP and thereafter incubated in the absence of the cyclic nucleotide for the assay of PAH transport, cGMP inhibited PAH accumulation in the slices in a dose-dependent fashion. Pretreatment of the slices with dibutyryl cGMP induced no inhibition of PAH uptake, but that with the combination of cGMP and butyrate inhibited the accumulation to the same extent as did cGMP. Guanosine and GMP had no effect on PAH accumulation in the slices. Inhibitory effect of cGMP on PAH accumulation was not due to the changes of electrolyte content and water distribution in the slices. The slices pretreated with cGMP at 0.degree. C failed to decrease PAH accumulation. cGMP had no effect on organic cation (tetraethylammonium, TEA) transport in the slices. cGMP may be able to modulate the rat renal transport system for PAH although this study does not explain the mechanism of inhibition of PAH transport by the cyclic nucleotide.

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