Component A of the methyl coenzyme M methylreductase system of Methanobacterium: resolution into four components.
- 1 April 1983
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 80 (8) , 2151-2155
- https://doi.org/10.1073/pnas.80.8.2151
Abstract
Component A, the oxygen-sensitive protein fraction of the methyl coenzyme M methylreductase system of Methanobacterium thermoautotrophicum, has been stabilized and resolved into three protein fractions and one cofactor that are required to reconstitute component A activity. Component A1 is oxygen-stable and contains hydrogen-dependent deazaflavin (coenzyme F420)-reducing activity. Component A2 is acidic; components A2 and A3 are oxygen sensitive. The specific functions of each component in methyl group reduction are unknown. Resolution of component A revealed a new cofactor requirement of the methylreductase system for FAD. Hydrogen-dependent reduction of methyl coenzyme M to methane and coenzyme M, the terminal step of CO2 reduction by methanogenic bacteria, requires protein components A1, A2, A3, and C in addition to component B, FAD, ATP, and Mg2+.This publication has 19 references indexed in Scilit:
- Purification and properties of an 8-hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum.Journal of Biological Chemistry, 1982
- Component C of the methylreductase system of Methanobacterium.Journal of Biological Chemistry, 1981
- Membrane-bound flavin adenine dinucleotide in methanobacterium bryantiiBiochemical and Biophysical Research Communications, 1981
- Role of component C in the methylreductase system of Methanobacterium.Journal of Biological Chemistry, 1980
- Methyl coenzyme M reductase from Methanobacterium thermoautotrophicum. Resolution and properties of the components.Journal of Biological Chemistry, 1980
- A procedure for anaerobic column chromatography employing an anaerobic freter-type chamberAnalytical Biochemistry, 1980
- Methanogens: reevaluation of a unique biological group.1979
- Preparation of coenzyme M analogs and their activity in the methyl coenzyme M reductase system of Methanobacterium thermoautotrophicumBiochemistry, 1978
- Preparation, characterization, and chemical properties of the flavin coenzyme analogues 5-deazariboflavin, 5-deazariboflavin 5'-phosphate, and 5-deazariboflavin 5'-diphosphate, 5' → 5'-adenosine esterBiochemistry, 1976
- Hydrogen Ion Buffers for Biological Research*Biochemistry, 1966