The Relationship between the Hemagglutination-Inhibition Test and the Enzyme-Linked Immunosorbent Assay for the Detection of Antibody to Newcastle Disease
- 1 July 1990
- journal article
- research article
- Published by JSTOR
- Vol. 34 (3) , 585-587
- https://doi.org/10.2307/1591248
Abstract
An assay of 364 chicken serum samples for Newcastle disease virus antibodies determined that a commercial NDV enzyme-linked immunosorbent assay (ELISA) had a 98.2% sensitivity and a 91.7% specificity relative to the NDV HI test. The ELISA values regressed significantly (F = 930, df = 1/362, P < 0.001) on the hemagglutination-inhibition (HI) titers. The correlational coefficient was 0.85. For individuals, two tests can have the same result based upon chance alone. Kappa is a measure of agreement between two tests that corrects for this chance agreement. The kappa between the ELISA and HI test was calculated to be 0.84 (Z = 7.74, P = 0.00001), which indicates a highly significant agreement between the two tests.This publication has 4 references indexed in Scilit:
- Quantitative estimation of Newcastle disease virus antibody levels in chickens and Turkeys by ElisaAvian Pathology, 1989
- An enzyme-linked immunosorbent assay method for the simultaneous measurement of antibody titer to multiple viral, bacterial or protein antigensVeterinary Immunology and Immunopathology, 1985
- Antibody Response to Newcastle Disease Virus Given by Two Different Routes as Measured by ELISA and Hemagglutination-Inhibition Test and Associated Tracheal ImmunityAvian Diseases, 1985
- Optimizing the Enzyme-Linked Immunosorbent Assay for Evaluating Immunity of Chickens to Newcastle DiseasePublished by JSTOR ,1983