IDENTIFICATION OF THE MOUSE CHROMOSOMES BY MICRODENSITOMETRY

Abstract
Densitometric analysis was performed on mouse chromosomes from in vivo preparations stained with Giemsa after trypsinization. The characteristic curves obtained by analyzing the same chromosome after different durations of trypsinization suggest that a trypsinization step is not always necessary to get an analyzable banding pattern. The best results are obtained when the chromatids are long and closely appressed and by analyzing the positive photomicrographs.