Cloned synthetic lac operator DNA is biologically active
Open Access
- 1 October 1976
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 263 (5580) , 744-748
- https://doi.org/10.1038/263744a0
Abstract
A chemically synthesised duplex DNA fragment containing the sequence of the lac operator was cloned in E. coli using the vehicle pMB9. Clones containing lac-pMB9 hybrid DNA produced β-galactosidase constitutively and the hybrid DNA bound the lac repressor specifically.This publication has 14 references indexed in Scilit:
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