Mechanism of biosynthesis of 11R‐ and 12R‐hydroxyeicosatetraenoic acids by eggs of the sea urchin Strongylocentrotus purpuratus

Publisher Website
Google Scholar
Abstract
11(R)‐Hydroxyeicosatetraenoic acid [11(R)‐HETE] and 12(R)‐HETE are biosynthesized by eggs of the sea urchin S. purpuratus. We report here the isolation of the 11(R)‐ and 12(R)‐hydroperoxy‐eicosanoids from incubations of the desalted 30–50% (NH4)2SO4 fraction of the egg homogenate; biosynthesis required the addition of calcium but not NADPH. Egg 11‐ and 12‐HETE were formed from octadeuterated arachidonic acid without loss of geminal 2H from C11 or C12, thus revealing that 11‐ or 12‐keto intermediates are not involved in the biosynthesis. The results support the conclusion that egg 11(R)‐ and 12(R)‐HETE are synthesized by a lipoxygenase and not by an NADPH‐dependent cytochrome P450 monooxygenase mechanism.

This publication has 18 references indexed in Scilit: