Propagation of Certain Arthropod-Borne Viruses in Avian and Primate Cell Cultures

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Abstract
Summary: Multiplication in tissue culture of 21 arthropod-borne viruses representative of serogroups A, B and C was manifested by a consistency of plaque formation under an agar overlay. Cytopathic reactions in infected fluid cultures were not always apparent even though virus multiplication was detected. In comparative virus titrations in tissue cultures and infant mice, the plaque assay was found to be the most sensitive system. In general, group A viruses were found to multiply in both avian (chick embryo and Pekin duck kidney or embryo) and the primate (rhesus monkey kidney) cell systems with few exceptions. Group B viruses could be propagated in Pekin duck kidney or Pekin duck embryo cultures, but not in chick embryo or rhesus monkey kidney (except Ilheus), and the group C viruses produced plaques only in rhesus monkey kidney cell cultures.