Production, isolation and structure determination of a novel .BETA.-glucosidase inhibitor, cyclophellitol, from Phellinus sp.
- 1 January 1990
- journal article
- research article
- Published by Japan Antibiotics Research Association in The Journal of Antibiotics
- Vol. 43 (1) , 49-53
- https://doi.org/10.7164/antibiotics.43.49
Abstract
In the course of our screening of .beta.-glucosidase inhibitor, a culture filtrate of a mushroom, Phellinus sp. strongly inhibited the enzyme activity. The active substance was isolated through charcoal separation, column chromatography and crystallization. Spectroscopic and crystallographic analysis revealed that it had a novel cyclitol structure, (1S,2R,3S,4R,5R,6R)-5-hydroxymethyl-7-oxabicyclo[4,1,0]heptane-2,3,4-triol, and we named it cyclophellitol. It inhibited almond-derived .beta.-glucosidase with an IC50 of 0.8 .mu.g/ml.This publication has 3 references indexed in Scilit:
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