Lens epithelium-derived growth factor (LEDGF) delays photoreceptor degeneration in explants of rd/rd mouse retina
- 1 September 2001
- journal article
- research article
- Published by Wolters Kluwer Health in NeuroReport
- Vol. 12 (13) , 2951-2955
- https://doi.org/10.1097/00001756-200109170-00039
Abstract
Lens epithelium derived growth factor (LEDGF) has been shown to rescue embryonic chick photoreceptor cells from serum starvation and heat stress, light damaged photoreceptor cells in Lewis rats, and photoreceptor cells in RCS rats. The aim of our study is to study the rescue effect of LEDGF on photoreceptor cells in the rd/rd mouse using our long-term serum free organ culture. At the end of this culture period of 21–26 days LEDGF treated rd mouse retina showed an increased photoreceptor survival compared to the untreated controls. LEDGF has no effect on expression and localization of opsin and arrestin in the rod photoreceptor cells when RPE is present. The protective potency of LEDGF on the retinal photoreceptor cells is similar to that of BDNF. LEDGF is known to activate heat shock proteins (Hsps) and the elevated Hsps are also reported to suppress apoptosis.Keywords
This publication has 18 references indexed in Scilit:
- LEDGF Binds to Heat Shock and Stress-Related Element to Activate the Expression of Stress-Related GenesBiochemical and Biophysical Research Communications, 2001
- Growth Factors in Combination, but Not Individually, Rescue rd Mouse Photoreceptors in Organ CultureExperimental Neurology, 2000
- Lens epithelium-derived growth factor (LEDGF/p75) and p52 are derived from a single gene by alternative splicingGene, 2000
- Presence of a pre-apoptotic complex of pro-caspase-3, Hsp60 and Hsp10 in the mitochondrial fraction of Jurkat cellsThe EMBO Journal, 1999
- Management of Hereditary Retinal DegenerationsSurvey of Ophthalmology, 1999
- Isolation of cDNAs encoding novel transcription coactivators p52 and p75 reveals an alternate regulatory mechanism of transcriptional activationThe EMBO Journal, 1998
- Hepatoma-Derived Growth Factor Belongs to a Gene Family in Mice Showing Significant Homology in the Amino TerminusBiochemical and Biophysical Research Communications, 1997
- Photoreceptor-specific protein expression of mouse retina in organ culture and retardation ofrddegenerationin vitroby a combination of basic fibroblast and nerve growth factorsCurrent Eye Research, 1993
- Binding sites of photoreceptor-specific antibodies COS-1, OS-2 and AOCurrent Eye Research, 1993
- Retinal degeneration in the rd mouse is caused by a defect in the β subunit of rod cGMP-phosphodiesteraseNature, 1990