Enzyme linked immunosorbent assay for rubella antibodies: a simple method of antigen production. A preliminary report
Open Access
- 1 August 1995
- journal article
- Published by FapUNIFESP (SciELO) in Revista do Instituto de Medicina Tropical de São Paulo
- Vol. 37 (4) , 357-359
- https://doi.org/10.1590/s0036-46651995000400013
Abstract
A simple method of rubella antigen production by treatment with sodium desoxycholate for use in enzyme immunoassay (IMT-ELISA) is presented. When this assay was compared with a commercial test (Enzygnost-Rubella, Behring), in the study of 108 sera and 118 filter paper blood samples, 96.9% (219/226) overall agreement and correlation coefficient of 0.90 between absorbances were observed. Seven samples showed discordant results, negative by the commercial kit and positive by our test. Four of those 7 samples were available, being 3 positive by HI.Keywords
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