Parameters of field inversion gel electrophoresis for the analysis of pox virus genomes
- 1 January 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 16 (10) , 4239-4252
- https://doi.org/10.1093/nar/16.10.4239
Abstract
The effects of variation in the lengths of forward and reverse pulses, voltage gradient, gel concentration and gel temperature on the mobility of DNA molecules in agarose gels during field inversion gel electrophoresis (FIGE) have been determined. A curve, which best fits the empirical data, is presented and allows the choice of pulse conditions and voltage gradient most suitable for the resolution of molecules of chosen size. The use of FIGE in the analysis and direct mapping of large virus genomes is illustrated using vaccinia virus DNA.Keywords
This publication has 14 references indexed in Scilit:
- Mapping the limits of the human pseudoautosomal region and a candidate sequence for the male-determining geneNature, 1987
- Megabase-Scale Mapping of the HLA Gene Complex by Pulsed Field Gel ElectrophoresisScience, 1987
- Ramped field inversion gel electrophoresis: a cautionary noteNucleic Acids Research, 1987
- An electrophoretic karyotype forSchizosaccharomyces pombeby pulsed field gel electrophoresisNucleic Acids Research, 1987
- A model for the separation of large DNA molecules by crossed field gel electrophoresisNucleic Acids Research, 1987
- Electrophoretic Separations of Large DNA Molecules by Periodic Inversion of the Electric FieldScience, 1986
- Chromosome rearrangements in trypanosoma bruceiPublished by Elsevier ,1984
- Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresisNucleic Acids Research, 1984
- Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresisCell, 1984
- Conservation and Variation in Orthopoxvirus Genome StructureJournal of General Virology, 1979