Synthesis of RNA by Separated Heads and Tails from Bovine Spermatozoa

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Abstract
Highly purified populations of heads and tails obtained from normal bovine spermatozoa and from spermatozoa carrying the decapitated sperm defect synthesize RNA when incubated in an assay mixture for RNA polymerase. Both fractions utilize endogenous templates and demonstrate maximal activity in the presence of MgCl2, 2-mercaptoethanol and all 4 ribonucleoside triphosphates. Although heads and tails share many similar reaction parameters, the synthesis in the 2 fractions can be distinguished by the following criteria: temperature optimum; MgCl2 optimum; inhibition by atractyloside (1-10 mM); inhibition by .alpha.-amanitin (1-5 .mu.g/ml); and inhibition by rifamycin SV (10-40 .mu.g/ml).

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