Abstract
A new method is described for establishing across living cells very steep conc. gradients of chemical substances in soln., or for establishing temp. gradients. The cells are embedded in a thin jelly membrane that separates 2 different flowing solns. When fertilized Fucus eggs, embedded in a thin agar membrane, are reared with sea water on one side and 3-5-5 [image] dinitrophenol in sea water on the other side of the membrane, a high proportion forms rhizoids on the sides toward the higher conc. of dinitrophenol.