Observations on bud formation and proliferations on cytokinin-treated root segments of Ophioglossum petiolatum

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Abstract
Root segments of the fern Ophioglossum petiolatum with either an intact root apex or with the apex removed were treated with distilled water (control) or a range of concentrations of kinetin or benzyladenine in aqueous solution. Buds initiated on segments treated with distilled water or low concentrations of cytokinins had an apical meristem consisting of an apical cell with derivatives and a few leaf primordia located immediately beneath an air cavity formed by the lysis of cortical cells of the parent root. There was little cortical proliferation associated with the initiation of these buds. However, application of cytokinins at relatively high concentrations induced considerable proliferation of root tissue and a concomitant organization of numerous apical meristems in this tissue. Each induced meristem was structurally similar to those initiated on control root segments or those treated with low concentrations of cytokinins with the exception that leaf primordia were not as readily visible. Groups of tracheid-like cells were present in the callus-like outgrowths and, in root segments treated with 10.0 mg/l kinetin, large starch-filled parenchyma cells were evident at the periphery of the proliferations.

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