RELEASE OF ESTROGEN-INDUCED GLYCOPROTEIN WITH A MOLECULAR-WEIGHT OF 52,000 BY BREAST-CANCER CELLS IN PRIMARY CULTURE

Google Scholar
  • 1 January 1983
    • journal article
    • research article
    • Vol. 43  (4) , 1861-1868
Abstract
In an attempt to find estrogen-specific responses in breast cancer, a primary cell culture was established from metastatic pleural effusions of breast cancer and the proteins released into the culture medium were analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The synthesis of a MW 52,000 glycoprotein which is released by metastatic breast cancer cells in primary cultures is stimulated by estradiol in 4 of 6 patients. This protein is similar to the MW 52,000 protein of MCF7 cells on the basis of its mobility in 1- and 2-dimensional gel electrophoresis [the MW of this protein was originally found to be 46,000; it is closer to 52,000 using labeled proteins from New England Nuclear as molecular weight markers], its immunoprecipitation by antisera raised against the MW 52,000 protein, and its binding to concanavalin A. Similar to some breast cancer cell lines, some metastatic breast cancers synthesize a MW 52,000 glycoprotein which is regulated by estrogens and exported from the cells into the medium. This study also shows that some primary cultures established from metastatic breast cancer remain responsive to estradiol in vitro for the synthesis of specific proteins. More clinical studies are needed to prove the interest to the MW 52,000 secreted protein as an additional marker of the hormone responsiveness of breast cancer.

This publication has 1 reference indexed in Scilit: