Enhancement of in vitro erythropoiesis by peripheral blood mononuclear cells from allogeneic marrow recipients in the early post‐transplant period

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Abstract
The effect of peripheral blood mononuclear cells (PBMCs) from bone marrow recipients on in vitro growth of erythroid burst‐forming units (BFU‐E) was studied. PBMCs were obtained from 5 allogeneic, 1 syngeneic and 1 autologous bone marrow recipient(s) at different intervals after transplantation. The number of BFU‐E was significantly increased when donor bone marrow cells were co‐cultured with PBMCs obtained from allogeneic marrow recipients in the early post‐transplant period. No effect was observed using PBMCs obtained in the later post‐transplant period, PBMCs from a syngeneic marrow recipient, or PBMCs from an autologous marrow recipient. The BFU‐E enhancing activity was present among T cells and was abolished by treating them with OKT3 or OKT4 antibody and complement. These observations suggest that chimeric T lymphocytes, probably of the helper/inducer subset, from allogeneic marrow recipients in the early post‐transplant period have a potent enhancing effect on in vitro erythropoiesis.