PREPARATION OF TRITIATED α‐MELANOTROPIN WITH HIGH SPECIFIC RADIOACTIVITY
- 1 April 1981
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 17 (4) , 514-518
- https://doi.org/10.1111/j.1399-3011.1981.tb02022.x
Abstract
α-Metanotropin (α-MSH) was iodinated and 3,5-diiodotyr2 -α-MSH was isolated by reverse phase high performance liquid chromatography (HPLC). Catalytic dehalogenation of the diiodo derivative in the presence of tritium resulted in the formation of 3,5-ditritiotyr2 -α-MSH. The tritiated peptide was purified by ion exchange and partition chromatography. The radioactive peptide was found to be homogeneous and identical to α-MSH by paper electrophoresis and HPLC. The tritiated α-MSH stimulated lipolysis in rabbit adipocytes nearly as well as α-MSH. The specific radioactivity of tritiated α-MSH was 42 Ci/mmol or 73% of the theoretical value.Keywords
This publication has 17 references indexed in Scilit:
- ISOLATION OF α-MELANOTROPIN AND N, O-DIACETYLSERINE1-α-MELANOTROPIN FROM PORCINE PITUITARY EXTRACTSInternational Journal of Peptide and Protein Research, 1981
- The presence of two α-MSH positive cell groups in rat hypothalamusEuropean Journal of Pharmacology, 1979
- Iodination associated inactivation of β-melanocyte stimulating hormoneBiochemical and Biophysical Research Communications, 1979
- PREPARATION AND PROPERTIES OF TRITIATED HUMANβ‐ENDORPHIN WITH HIGH SPECIFIC RADIOACTIVITYInternational Journal of Peptide and Protein Research, 1978
- Preparation and characterization of specifically tritiated adrenocorticotropinBiochimica et Biophysica Acta (BBA) - General Subjects, 1977
- Synthese von [D‐Alanin1, 4′ ‐azido‐3′, 5′ ‐ditritio‐L‐phenylalanin2, norvalin4]α‐melanotropin als «Photoaffinitätsprobe» für Hormon‐Rezeptor‐WechselwirkungenHelvetica Chimica Acta, 1976
- Labelled polypeptides. v. synthesis of α-melanotropin labelled with tritium on the tyrosine residue, via the dibromo intermediateJournal of Labelled Compounds and Radiopharmaceuticals, 1976
- Comparison of the steroidogenic and melanotropic activities of corticotropin, α-melanotropin and analogs with their lipolytic activities in rat and rabbit adipocytesBiochimica et Biophysica Acta (BBA) - General Subjects, 1976
- Divergent effects of adrenocorticotropin and melanotropin on isolated rat and rabbit adipocytesBiochimica et Biophysica Acta (BBA) - General Subjects, 1976
- Labelled polypeptides, III. Synthesis of α-melanotropin labelled with tritium on the tyrosine residueJournal of Labelled Compounds and Radiopharmaceuticals, 1974