REGULATION OF SOME FUNCTIONS OF GRANULOCYTES BY ZINC OF PROSTATIC FLUID AND PROSTATE TISSUE

  • 1 January 1976
    • journal article
    • research article
    • Vol. 88  (4) , 640-648
Abstract
O2 consumption, phagocytosis and activity of hexosomonophosphate shunt (HMS) of dog and human polystyrene latex-activated polymorphonuclear leukocytes were inhibited by normal and infected human prostatic fluid (PF) and prostate extract; resting cells were not affected. Granulocytes incubated in 7% PF increased 5 times their Zn content. Inorganic Zn added to synthetic medium which contained activated granulocytes also inhibited O2 consumption and phagocytosis of yeast particles in a concentration-dependent manner; resting cells were not affected. Incubation of PF with CaNa2 EDTA abolished the inhibitory effect of PF; extensive dialysis of PF against this chelating agent reduced the inhibition by 1/2. After the PF was dialyzed against saline, the low molecular weight dialysate retained inhibitory effects. Extracts from dog liver, spleen and human saliva were not inhibitory. Polyamines, which next to Zn are also present in PF in high concentrations, had no effect on granulocytes, O2 consumption and HMS activity. Human and dog PF were inhibitory to human or dog granulocytes, respectively. Besides granulocytes, rat peritoneal macrophages were inhibited by PF. PF and extract from prostate inhibit various functions of phagocytes mainly because of high concentration of Zn. The existence of other, large molecular weight heat-resistant substances in PF which contribute to Zn effect cannot be ruled out. [Prostatitis may become chronic due to a lack of antibacterial substances.].

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