Amino Acid Sequence of the Blood Group Mg-Specific Major Human Erythrocyte Membrane Sialoglycoprotein

Abstract
The structure of the N-terminal 21 residues of the blood group Mg-specific major human erythrocyte membrane sialoglycoprotein was investigated, using tryptic MgM peptides and secondary fragments prepared by staphylococcal V8 protease treatment. The sequence Leu-Ser-Thr-Asn-Glu was obtained for the N-terminal 5 residues. The Mg gene appears to have evolved from a Thr .fwdarw. Asn mutation of an N allele. This alteration prevented the glycosylation of the amino acids at the 2nd and 3rd positions.

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