Microsomal 12α‐Hydroxylation of 7α‐[12α,12β‐2H2]Hydroxy‐4‐cholesten‐3‐one
- 1 February 1975
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 51 (1) , 137-143
- https://doi.org/10.1111/j.1432-1033.1975.tb03914.x
Abstract
The synthesis of 7alpha-[12alpha, 12beta-2 H2]hydroxy-4-cholesten-3-one is described. It was shown with different techniques that this compound was 12alpha-hydroxylated by the microsomal fraction of a rat liver homogenate without marked isotope effect, indicating that cleavage of the C--H bond is not the rate-limiting step in this hydroxylation. The rate of 12alpha-hydroxylation was decreased by about 20% when performed in a medium containing deuterated water. The findings were discussed with reference to the specific properties of the 12alpha-hydroxylating system and to the results of previous studies on rate-limiting step in microsomal hydroxylation of steroids.Keywords
This publication has 23 references indexed in Scilit:
- 12α-Hydroxylation of 7α-hydroxy-4-cholesten-3-one by a reconstituted system from rat liver microsomesBiochemical and Biophysical Research Communications, 1973
- On the Rate-Limiting Step in Microsomal Hydroxylation of SteroidsEuropean Journal of Biochemistry, 1972
- On the rate-limiting step in ω-hydroxylation of lauric acidBiochemical and Biophysical Research Communications, 1972
- Effect of carbon monoxide and phenobarbital on hydroxylation of bile acids by rat liver microsomesFEBS Letters, 1969
- Effect of drug substrates on the reduction of hepatic microsomal cytochrome P-450 by NADPHBiochemical and Biophysical Research Communications, 1968
- The electron transport mechanism associated with 12α-hydroxylation of C27 steroidsBiochemical and Biophysical Research Communications, 1968
- On the Role of Delta4-3alpha-Hydroxysterols in the Biosynthesis of Bile Acids. Bile Acid and Steroids. 191.Acta Chemica Scandinavica, 1967
- The Dehydrogenation of Corticosteroids with Chloranil1Journal of the American Chemical Society, 1960
- Cholesterol and Companions. VII. Steroid DibromidesJournal of the American Chemical Society, 1953
- 122. Substituted ketocholanic acidsJournal of the Chemical Society, 1939