Bovine serum albumin: characterization of a fatty acid binding site on the N-terminal peptic fragment using a new spin-label

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Abstract
A new spin-label, 4-(L-glutamo)-4''-[(1-oxy-2,2,5,5-tetramethyl-3L-pyrrolidinyl)amino]-3,3''-dinitrodiphenyl sulfone, is shown to bind to 1 high-affinity binding site on bovine serum albumin [BSA] (K = 5 .times. 104 M-1, n = 1). Analysis of the binding of the spin-label to the amino-terminal half (peptic fragment PB) and the carboxy-terminal half (peptic fragment PA) of BSA, and their complex (PA-PB), indicates that the spin-label binds to a long-chain fatty acid binding site located on PB. The usefulness of the novel specificity of the spin-label in characterizing this binding site is discussed.