Inhibition of Postsynaptic PKC or CaMKII Blocks Induction But Not Expression of LTP
- 25 August 1989
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 245 (4920) , 862-866
- https://doi.org/10.1126/science.2549638
Abstract
Long-term potentiation (LTP) of synaptic transmission is a widely studied cellular example of synaptic plasticity. However, the identity, localization, and interplay among the biochemical signals underlying LTP remain unclear. Intracellular microelectrodes have been used to record synaptic potentials and deliver protein kinase inhibitors to postsynaptic CA1 pyramidal cells. Induction of LTP is blocked by intracellular delivery of H-7, a general protein kinase inhibitor, or PKC(19-31), a selective protein kinase C (PKC) inhibitor, or CaMKII(273-302), a selective inhibitor of the multifunctional Ca2+-calmodulin-dependent protein kinase (CaMKII). After its establishment, LTP appears unresponsive to postsynaptic H-7, although it remains sensitive to externally applied H-7. Thus both postsynaptic PKC and CaMKII are required for the induction of LTP and a presynaptic protein kinase appears to be necessary for the expression of LTP.Keywords
This publication has 41 references indexed in Scilit:
- Expression of a multifunctional Ca2+/calmodulin-dependent protein kinase and mutational analysis of its autoregulationPublished by Elsevier ,1989
- Postsynaptic Calcium Is Sufficient for Potentiation of Hippocampal Synaptic TransmissionScience, 1988
- Synaptic memory moleculesNature, 1988
- Phorbol ester-induced synaptic potentiation differs from long-term potentiation in the guinea pig hippocampus in vitroNeuroscience Letters, 1988
- Protein Kinase C Contains a Pseudosubstrate Prototope in Its Regulatory DomainScience, 1987
- Activation of protein kinase C augments evoked transmitter releaseNature, 1987
- Translocation of Protein Kinase C Activity May Mediate Hippocampal Long-Term PotentiationScience, 1986
- Selective impairment of learning and blockade of long-term potentiation by an N-methyl-D-aspartate receptor antagonist, AP5Nature, 1986
- Phorbol ester and calcium act synergistically to enhance neurotransmitter release by brain neurons in cultureFEBS Letters, 1985
- Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide-dependent protein kinase and protein kinase CBiochemistry, 1984