Estimation of Haemoglobin by the Alkaline Haematin Method

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Abstract
The alkaline hematin method of Wu (1922) is superior to other methods because it is reasonably accurate, simple and measures all forms of Hb. Alkaline hematin forms a true soln. and is easily prepd. from Hb. and from cryst. hematin. 16 samples of cryst. hematin were prepd. from ox and human blood and the Fe content of each detd. The intensity of color was proportional to the conc. of hemin Fe and impurities did not influence the color. If a 1 in 100 dilution of blood in N/10 NaOH is placed in a boiling water bath for 5 min. all the alkali-resistant and inactive forms of Hb. are converted into alkaline hematin.

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