Prodigiosin 25‐C uncouples vacuolar type H+‐ATPase, inhibits vacuolar acidification and affects glycoprotein processing
- 6 February 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 359 (1) , 53-59
- https://doi.org/10.1016/0014-5793(94)01446-8
Abstract
Prodigiosin 25-C inhibited the accumulation of 3-(2,4-dinitroanilino)-3'-amino-N-methyldipropylamine and acridine orange in the acidic compartments of baby hamster kidney cells with little perturbation of cellular ATP levels. In rat liver lysosomes, prodigiosin 25-C inhibited the proton pump activity with an IC50 of approximately 30 nM, but did not affect ATPase activity up to 1 microM. It also delayed the transport of vesicular stomatitis virus G protein and induced a drastic swelling of Golgi apparatus and mitochondria. These results indicate that prodigiosin 25-C raises the pH of acidic compartments through inhibition of the proton pump activity of vacuolar type H(+)-ATPase, thereby causing the functional and morphological changes to the Golgi apparatus.Keywords
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