RADIATION SENSITIVITY AND CYCLING STATUS OF MOUSE BONE-MARROW PROTHYMOCYTES AND DAY-8 COLONY-FORMING-UNITS SPLEEN (CFU-S)

  • 1 January 1983
    • journal article
    • research article
    • Vol. 11  (10) , 922-930
Abstract
Mouse bone marrow prothymocytes as determined in an in vivo thymus regeneration assay have an in vitro gamma radiation sensitivity which is different from that of spleen colony forming cells (CFUS). Determination of D0 [mean lethal dose] according to in vivo irradiation revealed similar but insignificant differences. Prothymocytes in normal bone marrow maintain a low but slightly different proliferative state as compared to CFUS, according to determination using the 3H-TdR [thymidine] suicide technique. In regenerating bone marrow prothymocytes were found to be sensitive to an inhibitory effect of in vitro incubation with cold thymidine. CFUS and normal bone marrow prothymocytes were not affected by cold thymidine. Taking into account the cold thymidine effect it can be concluded that prothymocytes and CFUS in regenerating bone marrow are fully in cycle. These results are best explained when prothymocytes and CFUS are considered to be different cells.

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