A simple and rapid method for sequencing DNA
- 1 January 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 179 (1) , 34-36
- https://doi.org/10.1016/0014-5793(85)80185-x
Abstract
A simplified technique for DNA sequence analysis has been developed, based on modification of a previous method [(1980) Methods Enzymol. 65, 499‐560]. It employs an adsorptive immobilization of terminally labelled DNA on DEAE paper followed by G, A + G, C + T and C specific modification and cleavage reactions. This solid‐phase technique is faster and more convenient than the original method. The efficiency is comparable. The total processing time taken to produce cleaved fragments loaded on a gel is less than 2h.Keywords
This publication has 1 reference indexed in Scilit:
- [57] Sequencing end-labeled DNA with base-specific chemical cleavagesPublished by Elsevier ,2004