Cell division arrest induced by phorbol ester in CHO cells overexpressing protein kinase C-delta subspecies.
- 1 November 1992
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (21) , 10159-10163
- https://doi.org/10.1073/pnas.89.21.10159
Abstract
Several lines of CHO cells stably overexpressing protein kinase C (PKC) subspecies to various extents were established by the DNA-mediated transfer. Upon treatment with phorbol 12-myristate 13-acetate, the growth of the cells expressing the PKC-delta subspecies was markedly inhibited, whereas cell lines expressing PKC-alpha, PKC-beta II, and PKC-zeta subspecies were not significantly affected. Flow cytometric analysis indicated that all cell lines overexpressing PKC-delta subspecies accumulated in G2/M phase in response to phorbol 12-myristate 13-acetate. In these arrested cells, dikaryons were predominant, implying that phorbol ester-induced inhibition of cell division is specific to telophase. These results suggest PKC-delta subspecies may play a role in the normal cell cycle progression.Keywords
This publication has 36 references indexed in Scilit:
- Structure and properties of a ubiquitously expressed protein kinase C, nPKCδEuropean Journal of Biochemistry, 1991
- Failure of wild-type or a mutant form of protein kinase C-α to transform fibroblastsNature, 1991
- Mouse protein kinase C-.delta., the major isoform expressed in mouse hemopoietic cells: sequence of the cDNA, expression patterns, and characterization of the proteinBiochemistry, 1991
- Protein kinases and protooncogenes: biochemical regulators of the eukaryotic cell cycleBiochemistry, 1991
- Universal control mechanism regulating onset of M-phaseNature, 1990
- A mutant protein kinase C that can transform fibroblastsNature, 1989
- The molecular heterogeneity of protein kinase C and its implications for cellular regulationNature, 1988
- High Efficiency DNA-Mediated Transformation of Primate CellsScience, 1983
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970