Tenascin‐C and SF/HGF produced by myofibroblasts in vitro provide convergent proinvasive signals to human colon cancer cells through RhoA and Rac
- 14 April 2004
- journal article
- Published by Wiley in The FASEB Journal
- Vol. 18 (9) , 1016-1018
- https://doi.org/10.1096/fj.03-1110fje
Abstract
SPECIFIC AIMDynamic and reciprocal communication between epithelial and stromal compartments is crucial for cancer progression. Myofibroblasts are stromal cells abundantly present at the invasion front of primary colon tumors. Their potential role in the transition from the noninvasive to invasive phenotype is suggested by their appearance before the invasive stage of the tumor. We have tried to better understand the cellular and molecular interactions between colon cancer cells and myofibroblasts.PRINCIPAL FINDINGS1. Myofibroblasts and rTGF-β1-treated fibroblasts stimulate invasion of colon cancer cells into collagen and Matrigel in vitroMyofibroblasts, in contrast to fibroblasts isolated from the adjacent normal colon of the same patient, stimulated invasion into collagen of HCT-8/E11, HCT-8/E11R1, and SW480 colon cancer cells in a 24 h assay. The presence of myofibroblasts in collagen changed the morphotype of colon cancer cells from round to elongated, with formation of cellular extensions. Invasion i...Keywords
Funding Information
- Kom op tegen Kanker
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