Guanidine-sensitive Na+ accumulation by poliovirus-infected HeLa cells

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Abstract
The Na+ content of poliovirus-infected [human cervical carcinoma] HeLa S3 cells increased during the late phase of virus replication, after virus inhibition of host cell protein synthesis and in coincidence with late viral functions. Guanidine HCl blocked the rise in Na+ content, whereas the antiguanidine agent choline fully reversed the guanidine block. Expression of 1 or more late viral functions was essential for Na+ accumulation to occur because accumulation was inhibited by cycloheximide or guanidine added to the infected culture during the late phase. Increased permeability of infected cell membrane rather than inhibition of cellular Na+-K+ ATPase activity appears to be primarily responsible for Na+ accumulation by virus-infected cells.