Accumulation of 10-kilobase DNA replication intermediates in cells treated with 3-aminobenzamide.
- 1 January 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (1) , 104-108
- https://doi.org/10.1073/pnas.82.1.104
Abstract
During eukaryotic DNA synthesis there is formation of, in addition to Okazaki fragments, discrete 10-kilobase (kb) DNA replication intermediates. The ligation of 10-kb DNA replication intermediates to high MW DNA was investigated using the drug 3-aminobenzamide, an inhibitor of poly(ADP-ribose) synthetase. In human melanoma cells treated with this inhibitor, there is an accumulation of 10-kb DNA. In cells treated with aphidicolin, which inhibits DNA polymerase .alpha., there is continued ligation of 10-kb DNA to high MW DNA. Using sequential treatment with aphidicolin and 3-aminobenzamide, the conversion of radiolabeled Okazaki fragments into 10-kb intermediates can be observed. The 10-kb DNA pieces are not ligated to high MW DNA in the presence of 3-aminobenzamide. Apparently, functioning poly(ADP-ribose) synthetase is necessary for the ligation process.This publication has 21 references indexed in Scilit:
- Aphidicolin inhibits the synthesis and joining of short DNA fragments but not the union of 10-kilobase DNA replication intermediates.Proceedings of the National Academy of Sciences, 1983
- Activation of DNA ligase by poly(ADP-ribose) in chromatin.Proceedings of the National Academy of Sciences, 1983
- New views of the biochemistry of eucaryotic DNA replication revealed by aphidicolin, an unusual inhibitor of DNA polymerase αCell, 1981
- (ADP-ribose)n participates in DNA excision repairNature, 1980
- Nuclear protein modification and chromatin substructure. 2. Internucleosomal localization of poly(adenosine diphosphate-ribose) polymeraseBiochemistry, 1978
- Delayed flow-through cytoplasm of newly synthesized Balbiani ring 75S RNACell, 1978
- Poly (ADP-Ribose) and ADP-Ribosylation of ProteinsAnnual Review of Biochemistry, 1977
- Poly(adenosine diphosphate-ribose) polymerase: the distribution of a chromosome-associated enzyme within the chromatin substructureBiochemistry, 1977
- Changes in poly(adenosine diphosphate-ribose) and poly(adenosine diphosphate-ribose) polymerase in synchronous HeLa cellsBiochemistry, 1976
- Natural occurrence of poly(ADP-ribosyl) histones in rat liver.Proceedings of the National Academy of Sciences, 1975