STUDY OF THE FLOCCULATION OFSACCHAROMYCES DIASTATICUS NCYC 625

Abstract
Yeast flocculation presents a great interest for the industry of fermentation but its mechanism is still not fully understood. In order to enlighten this mechanism, the flocculation of Saccharomyces diastaticus NCYC 625 was studied. As with other Saccharomyces strains, the effects of different factors affecting flocculation and deflocculation (pH, temperature, medium, EDTA, cations…) suggest a lectin-like binding between adjoining cells. The genetic determinism of flocculation is nuclear and not mitochondrial. Although Saccharomyces diastaticus NCYC 625 could be classified in the FLO1 phenotype according to Stratford and Assinder42, allelism tests show that the gene involved in the flocculation control is not allelic with FLO1 or FLO5 and possibly different from FLO8.