The Histochemical Significance of Staining Polysaccharide Sulfate Esters with Gentian Violet

1 January 1956

journal article
research article
Published by Taylor & Francis in Stain Technology

Vol. 31 (2) , 71-75
https://doi.org/10.3109/10520295609113779

Abstract

The technic recommended is: Fix in any fixative, dehydrate in alcohol, embed, and cut paraffin sections. Stain 24 hr or overnight in 1:250,000 or 1:1,280,000 gentian violet, blot with filter paper, decolorize 5 min in an aniline-xylene mixture, clear in xylene, and cover in balsam. Results: Polysaccharide sulfate ester and heparin are stained but hyaluronic acid is not. Polysaccharide sulfate ester and heparin are resistant to hyaluronidase. The differentiating action of the method is based on the strong basophilia of polysaccharide sulfate ester and the staining obtained is specific.

Keywords

This publication has 3 references indexed in Scilit:

HISTOLOGICAL AND HISTOCHEMICAL STUDIES ON POLKISSEN (ZIMMERMANN)
The Japanese Journal of Urology, 1953
HISTOCHEMICAL STUDIES ON THE PATHOGENESIS OF FIBRINOID
1949
Staining reactions of the mucoproteins
The Anatomical Record, 1940