Polynucleotide · Ribosomal‐Protein Complexes and Their Decoding Properties
Open Access
- 1 May 1982
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 124 (2) , 275-281
- https://doi.org/10.1111/j.1432-1033.1982.tb06588.x
Abstract
Polyadenylic acid, polycytidylic acid, polyuridylic acid or phage MS2 RNA, immobiliLed on Sepharose, form a complex with Escherichiu coli ribosomal proteins. Regardless of their particular nucleotide composition, all four polynucleotides bind an invariable set of proteins consisting of S1, S3, S4, S5, S9. S13, L2 and L17. We found that these polynucleotide. protein complexes bind tRNA. Furthermore, it was possible to show that the poly(A). protein and poly(U). protein complexes select efficiently their cognate tRNAs, tRNALys and tRNAPhe respectively. This important functional property of the polynucleotide. protein complexes suggests that these ribosomal proteins belong in the ribosome to a functional domain responsible for the decoding of mRNA.This publication has 34 references indexed in Scilit:
- Crosslinking of chemically reactive A-U-G analogs to ribosomal proteins within initiation complexesJournal of Molecular Biology, 1979
- Studies on the role of amino acid residues 31 through 46 of ribosomal protein S4 in the mechanism of 30 S ribosome assemblyJournal of Molecular Biology, 1978
- Nucleic acid binding properties of Escherichia coli ribosomal protein S1Journal of Molecular Biology, 1978
- Identification of several proteins involved in the messenger RNA binding site of the 30 S ribosome by inactivation with 2-methoxy-5-nitrotroponeJournal of Molecular Biology, 1977
- The interaction of transfer ribonucleic acid with 50 S ribosomal subunit proteinsFEBS Letters, 1977
- The codon binding site of the Escherichia coli ribosome as studied with a chemically reactive A-U-G analogJournal of Molecular Biology, 1975
- Protein neighborhoods in the 30S ribosomal subunit of Escherichia coliFEBS Letters, 1975
- Identification of proteins functionally altered by chemical modification of the transfer RNA and polyuridylic acid binding sites of 30 S ribosomal subunitsJournal of Molecular Biology, 1975
- Functional heterogeneity of the 30 s ribosomal subunit of Escherichia coli: III. Requirement of protein s1 for translationJournal of Molecular Biology, 1974
- Conformational changes in ribosomal subunits following dissociation of the Escherichia coli 70 S ribosomeJournal of Molecular Biology, 1973