A Japanese Family with Two Sisters Apparently Homozygous for Mk

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Abstract
Two Japanese sisters with consanguineous parents have M‐N‐ En(a‐) Wr(a‐b‐) S‐s‐U‐ red cells and are therefore apparently homozygous for Mk; the third reported family with members of this genotype. The serum of the proposita (ORCMK) contained anti‐EnaTS, anti‐EnaFR and possibly anti‐Wrbwhereas the serum of her MkMksister contained no atypical antibodies. Total absence of sialoglycoproteins α and δ from red cell membranes of an Mkhomozygote was demonstrated by lactoperoxidase‐catalysed radioiodination of accessible tyrosine residues with subsequent SDS polyacrylamide gel electrophoresis and autoradiography, and by use of a monoclonal antibody directed at the cytoplasmic portion of α‐sialoglycoprotein.