Reaction of vanadium(V) with thiols generates vanadium(IV) and thiyl radicals
- 1 October 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 271 (1-2) , 185-188
- https://doi.org/10.1016/0014-5793(90)80402-5
Abstract
The in vivo toxicity of vanadium(V) has been found to correlate with the depletion of cellular glutathione and related non-protein thiols. With a view to understanding the mechanism for this observation, we have investigated the oxidation of glutathione, cysteine N-acetylcysteine and penicillamine by vanadium(V), using electron spin resonance (ESR) and ESR spin trapping methodology. The spin trap used was 5,5-dimethyl-1-pyrroline 1-oxide (DMPO). It is found that the oxidation of these thiols by vanadium(V) generates the corresponding thiyl radicals and vanadium- (IV) complexes. The results suggest that free radical reactions play a significant role in the depletion of cellular thiols by vanadium(V) and hence in vanadium(V) toxicity.Keywords
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