Folding topology and DNA binding of the N‐terminal fragment of ada protein
Open Access
- 1 June 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 323 (3) , 252-256
- https://doi.org/10.1016/0014-5793(93)81351-y
Abstract
Three amino terminal fragments of Escherichia coli Ada protein (39 kDa) with different molecular masses (14 kDa, 16 kDa and 20 kDa) were prepared in large quantities from an E. coli strain harboring plasmids constructed for the overproduction of the truncated proteins. The three fragments can be methylated to an extent similar to that of the intact molecule. The methylated 16 kDa fragment specifically binds to the ada box on a DNA duplex. NMR analyses revealed that the 14 kDa fragment comprises two α‐helices and a β‐sheet with parallel and anti‐parallel mixed strands. A comparison of the 15N‐1H HMQC spectra of the fragments has led to the conclusion that this tertiary structure within the 14 kDa fragment is retained in the larger 16 kDa and 20 kDa fragments.Keywords
This publication has 18 references indexed in Scilit:
- Zinc binding by the methylation signaling domain of the Escherichia coli Ada proteinBiochemistry, 1992
- Applications of three- and four-dimensional heteronuclear NMR spectroscopy to protein structure determinationProgress in Nuclear Magnetic Resonance Spectroscopy, 1991
- [3] Predicting protein secondary structure based on amino acid sequencePublished by Elsevier ,1991
- Positive and negative regulation of transcription by a cleavage product of Ada proteinJournal of Molecular Biology, 1990
- A novel approach for sequential assignment of proton, carbon-13, and nitrogen-15 spectra of larger proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulinBiochemistry, 1990
- REGULATION AND EXPRESSION OF THE ADAPTIVE RESPONSE TO ALKYLATING AGENTSAnnual Review of Biochemistry, 1988
- Adaptive response of escherichia coli to alkylation damageEnvironmental and Molecular Mutagenesis, 1988
- Regulatory mechanisms for induction of synthesis of repair enzymes in response to alkylating agents: ada protein acts as a transcriptional regulator.Proceedings of the National Academy of Sciences, 1986
- The intracellular signal for induction of resistance to alkylating agents in E. coliCell, 1986
- Active site and complete sequence of the suicidal methyltransferase that counters alkylation mutagenesis.Proceedings of the National Academy of Sciences, 1985