Continuous determination of phenol, vitamin C, lysine and glucose in flowing solutions by means of an amperometric enzyme electrode
- 1 January 1981
- journal article
- Published by Institute of Organic Chemistry & Biochemistry in Collection of Czechoslovak Chemical Communications
- Vol. 46 (11) , 2871-2876
- https://doi.org/10.1135/cccc19812871
Abstract
A model apparatus is described for the continuous determination of compounds susceptible to catalytic oxidation by enzymes attached chemically to a membrane covering Clark's oxygen cell. The substrate concentration is estimated indirectly from the consumption of oxygen, which is proportional to the area under the curve registered at a constant potential of -0.65 V. A linear relation was found for phenol, ascorbic acid, L-lysine, glucose and polyamines. It is possible to analyze 15-30 samples in 1 h, depending on the flow rate of the carrier buffer.Keywords
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